col ii Search Results


sodium bicarbonate  (Chem Impex International)
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col ii from chicken sternum md biosciences bioproducts 804002-lyo  (MD Biosciences)
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arthritogenic antibodies specific for col ii epitopes  (MD Biosciences)
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collagen i gb11022−3 antibody  (Servicebio Inc)
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col ii protein expression  (Micromass UK Limited)
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Micromass UK Limited col ii protein expression
List of antibodies used for IHC of micromass cartilages.
Col Ii Protein Expression, supplied by Micromass UK Limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primer col ii a1 cat. no. qt00049518  (Qiagen)
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Qiagen primer col ii a1 cat. no. qt00049518
Comparative analysis of MSC and Chondrocyte markers in the tissue (D0) isolated from mild and severe OA regions. ( A ) The mRNA expression levels of CD105, CD166 and Notch 1 (MSC markers) in mild versus severe OA at D0; ( B ) The mRNA expression levels of Sox9, Acan and <t>Col</t> <t>II</t> (chondrocyte markers) in mild versus severe OA at D0. Mild OA is used as the control expression to normalized results.
Primer Col Ii A1 Cat. No. Qt00049518, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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col ii gb150010-100  (Servicebio Inc)
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Comparative analysis of MSC and Chondrocyte markers in the tissue (D0) isolated from mild and severe OA regions. ( A ) The mRNA expression levels of CD105, CD166 and Notch 1 (MSC markers) in mild versus severe OA at D0; ( B ) The mRNA expression levels of Sox9, Acan and <t>Col</t> <t>II</t> (chondrocyte markers) in mild versus severe OA at D0. Mild OA is used as the control expression to normalized results.
Col Ii Gb150010 100, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-col ii antibody  (Servicebio Inc)
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Comparative analysis of MSC and Chondrocyte markers in the tissue (D0) isolated from mild and severe OA regions. ( A ) The mRNA expression levels of CD105, CD166 and Notch 1 (MSC markers) in mild versus severe OA at D0; ( B ) The mRNA expression levels of Sox9, Acan and <t>Col</t> <t>II</t> (chondrocyte markers) in mild versus severe OA at D0. Mild OA is used as the control expression to normalized results.
Anti Col Ii Antibody, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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col ii  (Corning Life Sciences)
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Comparative analysis of MSC and Chondrocyte markers in the tissue (D0) isolated from mild and severe OA regions. ( A ) The mRNA expression levels of CD105, CD166 and Notch 1 (MSC markers) in mild versus severe OA at D0; ( B ) The mRNA expression levels of Sox9, Acan and <t>Col</t> <t>II</t> (chondrocyte markers) in mild versus severe OA at D0. Mild OA is used as the control expression to normalized results.
Col Ii, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse col ii  (Gallus BioPharmaceuticals)
90
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Comparative analysis of MSC and Chondrocyte markers in the tissue (D0) isolated from mild and severe OA regions. ( A ) The mRNA expression levels of CD105, CD166 and Notch 1 (MSC markers) in mild versus severe OA at D0; ( B ) The mRNA expression levels of Sox9, Acan and <t>Col</t> <t>II</t> (chondrocyte markers) in mild versus severe OA at D0. Mild OA is used as the control expression to normalized results.
Mouse Col Ii, supplied by Gallus BioPharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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col ii (col2a1, 250484, abbiotec)  (Abbiotec Inc)
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Comparative analysis of MSC and Chondrocyte markers in the tissue (D0) isolated from mild and severe OA regions. ( A ) The mRNA expression levels of CD105, CD166 and Notch 1 (MSC markers) in mild versus severe OA at D0; ( B ) The mRNA expression levels of Sox9, Acan and <t>Col</t> <t>II</t> (chondrocyte markers) in mild versus severe OA at D0. Mild OA is used as the control expression to normalized results.
Col Ii (Col2a1, 250484, Abbiotec), supplied by Abbiotec Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dir-labeled lnp-col ii-r nanoparticles  (AAT Bioquest)
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AAT Bioquest dir-labeled lnp-col ii-r nanoparticles
Comparative analysis of MSC and Chondrocyte markers in the tissue (D0) isolated from mild and severe OA regions. ( A ) The mRNA expression levels of CD105, CD166 and Notch 1 (MSC markers) in mild versus severe OA at D0; ( B ) The mRNA expression levels of Sox9, Acan and <t>Col</t> <t>II</t> (chondrocyte markers) in mild versus severe OA at D0. Mild OA is used as the control expression to normalized results.
Dir Labeled Lnp Col Ii R Nanoparticles, supplied by AAT Bioquest, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


List of antibodies used for IHC of micromass cartilages.

Journal: Bone Marrow Research

Article Title: A Simplified Method for the Aspiration of Bone Marrow from Patients Undergoing Hip and Knee Joint Replacement for Isolating Mesenchymal Stem Cells and In Vitro Chondrogenesis

doi: 10.1155/2016/3152065

Figure Lengend Snippet: List of antibodies used for IHC of micromass cartilages.

Article Snippet: Tuan laboratory showed that COL2A1 gene was expressed at day 14 and increased at days 21 and 28, the similar pattern of COL II protein expression we showed in micromass cartilages by IHC ( ).

Techniques: Plasmid Preparation

IHC of COL II (a) and COL I (b) in micromass cartilages. COL II expressed at weeks 2–4 (a), and COL I expressed at weeks 1–4 (week 3 not shown, b), in micromass cartilages. Articular cartilage of rat knee and human tracheal cartilage served as positive control for COL II; higher magnification of week 4 micromass cartilage shows that it is present in ECM (a). COL I is absent in native articular cartilage in positive control and is present in bone only, whereas COL I is expressed at low level in the bioengineered micromass cartilage (b). Micromass cartilage and rat tibia showed no staining when primary antibody was missing and only mouse IgG was present, which served as negative control (c). M, meniscus; AC, articular cartilage; B, bone; mm, micromass.

Journal: Bone Marrow Research

Article Title: A Simplified Method for the Aspiration of Bone Marrow from Patients Undergoing Hip and Knee Joint Replacement for Isolating Mesenchymal Stem Cells and In Vitro Chondrogenesis

doi: 10.1155/2016/3152065

Figure Lengend Snippet: IHC of COL II (a) and COL I (b) in micromass cartilages. COL II expressed at weeks 2–4 (a), and COL I expressed at weeks 1–4 (week 3 not shown, b), in micromass cartilages. Articular cartilage of rat knee and human tracheal cartilage served as positive control for COL II; higher magnification of week 4 micromass cartilage shows that it is present in ECM (a). COL I is absent in native articular cartilage in positive control and is present in bone only, whereas COL I is expressed at low level in the bioengineered micromass cartilage (b). Micromass cartilage and rat tibia showed no staining when primary antibody was missing and only mouse IgG was present, which served as negative control (c). M, meniscus; AC, articular cartilage; B, bone; mm, micromass.

Article Snippet: Tuan laboratory showed that COL2A1 gene was expressed at day 14 and increased at days 21 and 28, the similar pattern of COL II protein expression we showed in micromass cartilages by IHC ( ).

Techniques: Positive Control, Staining, Negative Control

IHC of COL X shows the presence of weak signal in week 3 and week 4 micromass cartilage (a), whereas apoptosis assays show no apoptosis in micromass cartilage (b). Mouse decalcified P1 limb showed hypertrophic chondrocytes zone (arrows) as positive control for COL X (a). Mouse involuting MG (mammary gland) at postpartum day 4 showed apoptotic cells in mammary epithelium (arrow) that served as positive control, whereas no apoptotic cell was found at week 4 in micromass cartilage compared with positive control. mm, micromass.

Journal: Bone Marrow Research

Article Title: A Simplified Method for the Aspiration of Bone Marrow from Patients Undergoing Hip and Knee Joint Replacement for Isolating Mesenchymal Stem Cells and In Vitro Chondrogenesis

doi: 10.1155/2016/3152065

Figure Lengend Snippet: IHC of COL X shows the presence of weak signal in week 3 and week 4 micromass cartilage (a), whereas apoptosis assays show no apoptosis in micromass cartilage (b). Mouse decalcified P1 limb showed hypertrophic chondrocytes zone (arrows) as positive control for COL X (a). Mouse involuting MG (mammary gland) at postpartum day 4 showed apoptotic cells in mammary epithelium (arrow) that served as positive control, whereas no apoptotic cell was found at week 4 in micromass cartilage compared with positive control. mm, micromass.

Article Snippet: Tuan laboratory showed that COL2A1 gene was expressed at day 14 and increased at days 21 and 28, the similar pattern of COL II protein expression we showed in micromass cartilages by IHC ( ).

Techniques: Positive Control

IHC showed that micromass cartilage expressed aggrecan and COL VI at all the time points (weeks 1–4, a, b). Articular cartilage of rat knee expressed aggrecan and served as positive control (a). Human tracheal cartilage served as positive control for COL VI and showed that COL VI is pericellular in location in native cartilage (b). Higher magnification of week 4 bioengineered micromass cartilage also shows the pericellular localization of COL VI (arrowheads, b). Rat knee (a) and micromass cartilage (b) were used as negative control with rabbit IgG polyclonal and without primary antibody. M, meniscus; AC, articular cartilage; B, bone; mm, micromass.

Journal: Bone Marrow Research

Article Title: A Simplified Method for the Aspiration of Bone Marrow from Patients Undergoing Hip and Knee Joint Replacement for Isolating Mesenchymal Stem Cells and In Vitro Chondrogenesis

doi: 10.1155/2016/3152065

Figure Lengend Snippet: IHC showed that micromass cartilage expressed aggrecan and COL VI at all the time points (weeks 1–4, a, b). Articular cartilage of rat knee expressed aggrecan and served as positive control (a). Human tracheal cartilage served as positive control for COL VI and showed that COL VI is pericellular in location in native cartilage (b). Higher magnification of week 4 bioengineered micromass cartilage also shows the pericellular localization of COL VI (arrowheads, b). Rat knee (a) and micromass cartilage (b) were used as negative control with rabbit IgG polyclonal and without primary antibody. M, meniscus; AC, articular cartilage; B, bone; mm, micromass.

Article Snippet: Tuan laboratory showed that COL2A1 gene was expressed at day 14 and increased at days 21 and 28, the similar pattern of COL II protein expression we showed in micromass cartilages by IHC ( ).

Techniques: Positive Control, Negative Control

Comparative analysis of MSC and Chondrocyte markers in the tissue (D0) isolated from mild and severe OA regions. ( A ) The mRNA expression levels of CD105, CD166 and Notch 1 (MSC markers) in mild versus severe OA at D0; ( B ) The mRNA expression levels of Sox9, Acan and Col II (chondrocyte markers) in mild versus severe OA at D0. Mild OA is used as the control expression to normalized results.

Journal: International Journal of Molecular Sciences

Article Title: Progenitor Cells from Cartilage: Grade Specific Differences in Stem Cell Marker Expression

doi: 10.3390/ijms18081759

Figure Lengend Snippet: Comparative analysis of MSC and Chondrocyte markers in the tissue (D0) isolated from mild and severe OA regions. ( A ) The mRNA expression levels of CD105, CD166 and Notch 1 (MSC markers) in mild versus severe OA at D0; ( B ) The mRNA expression levels of Sox9, Acan and Col II (chondrocyte markers) in mild versus severe OA at D0. Mild OA is used as the control expression to normalized results.

Article Snippet: Cells and tissue were tested on the expression of the following primers: CD105 (Cat. No. QT00013335, Qiagen, Paris, France), CD166 (Cat. No. Q100026824, Qiagen, Paris, France), Notch 1 (Cat. No. QT00001365, Qiagen, Paris, France), ACAN (Cat. No. QT00001365, Qiagen, Paris, France), Col II A1 (Cat. No. QT00049518, Qiagen, Paris, France).

Techniques: Isolation, Expressing, Control

Comparative analysis of MSC and Chondrocyte markers between tissue (D0) and proliferated cells (D14) in Mild OA. ( A ) The mRNA expression levels of CD105, CD166 and Notch 1 (MSC markers) at D14 versus D0; ( B ) The mRNA expression levels of Sox9, Acan and Col II (chondrocytes markers) at D14 versus D0. D0 is used as the control expression to normalise results. ** p < 0.01, *** p < 0.001, **** p < 0.0001, Student’s t -test ( n = 8 in Mild OA, n = 6 in severe OA).

Journal: International Journal of Molecular Sciences

Article Title: Progenitor Cells from Cartilage: Grade Specific Differences in Stem Cell Marker Expression

doi: 10.3390/ijms18081759

Figure Lengend Snippet: Comparative analysis of MSC and Chondrocyte markers between tissue (D0) and proliferated cells (D14) in Mild OA. ( A ) The mRNA expression levels of CD105, CD166 and Notch 1 (MSC markers) at D14 versus D0; ( B ) The mRNA expression levels of Sox9, Acan and Col II (chondrocytes markers) at D14 versus D0. D0 is used as the control expression to normalise results. ** p < 0.01, *** p < 0.001, **** p < 0.0001, Student’s t -test ( n = 8 in Mild OA, n = 6 in severe OA).

Article Snippet: Cells and tissue were tested on the expression of the following primers: CD105 (Cat. No. QT00013335, Qiagen, Paris, France), CD166 (Cat. No. Q100026824, Qiagen, Paris, France), Notch 1 (Cat. No. QT00001365, Qiagen, Paris, France), ACAN (Cat. No. QT00001365, Qiagen, Paris, France), Col II A1 (Cat. No. QT00049518, Qiagen, Paris, France).

Techniques: Expressing, Control

Comparative analysis of MSC and Chondrocytes markers between tissue (D0) and proliferated cells (D14) in severe OA. ( A ) The mRNA expression levels of CD105, CD166 and Notch 1 (MSC markers) at D14 versus D0; ( B ) The mRNA expression levels of Sox9, Acan and Col II (chondrocytes markers) at D14 versus D0. D0 is used as the control expression to normalized results. ** p < 0.01, Student’s t -test ( n = 8 in Mild OA, n = 6 in severe OA).

Journal: International Journal of Molecular Sciences

Article Title: Progenitor Cells from Cartilage: Grade Specific Differences in Stem Cell Marker Expression

doi: 10.3390/ijms18081759

Figure Lengend Snippet: Comparative analysis of MSC and Chondrocytes markers between tissue (D0) and proliferated cells (D14) in severe OA. ( A ) The mRNA expression levels of CD105, CD166 and Notch 1 (MSC markers) at D14 versus D0; ( B ) The mRNA expression levels of Sox9, Acan and Col II (chondrocytes markers) at D14 versus D0. D0 is used as the control expression to normalized results. ** p < 0.01, Student’s t -test ( n = 8 in Mild OA, n = 6 in severe OA).

Article Snippet: Cells and tissue were tested on the expression of the following primers: CD105 (Cat. No. QT00013335, Qiagen, Paris, France), CD166 (Cat. No. Q100026824, Qiagen, Paris, France), Notch 1 (Cat. No. QT00001365, Qiagen, Paris, France), ACAN (Cat. No. QT00001365, Qiagen, Paris, France), Col II A1 (Cat. No. QT00049518, Qiagen, Paris, France).

Techniques: Expressing, Control

Comparative analysis of MSC and Chondrocyte markers in proliferated cells (D14) isolated from mild and severe OA regions. ( A ) The mRNA expression levels of CD105, CD166 and Notch 1 (MSC markers) in mild versus severe OA at D14; ( B ) The mRNA expression levels of Sox9, Acan and Col II (chondrocytes markers) in mild versus severe OA at D148. Mild OA is used as the control expression to normalized results. * p < 0.05, ** p < 0.01, Student’s t -test ( n = 8 in Mild OA, n = 6 in severe OA).

Journal: International Journal of Molecular Sciences

Article Title: Progenitor Cells from Cartilage: Grade Specific Differences in Stem Cell Marker Expression

doi: 10.3390/ijms18081759

Figure Lengend Snippet: Comparative analysis of MSC and Chondrocyte markers in proliferated cells (D14) isolated from mild and severe OA regions. ( A ) The mRNA expression levels of CD105, CD166 and Notch 1 (MSC markers) in mild versus severe OA at D14; ( B ) The mRNA expression levels of Sox9, Acan and Col II (chondrocytes markers) in mild versus severe OA at D148. Mild OA is used as the control expression to normalized results. * p < 0.05, ** p < 0.01, Student’s t -test ( n = 8 in Mild OA, n = 6 in severe OA).

Article Snippet: Cells and tissue were tested on the expression of the following primers: CD105 (Cat. No. QT00013335, Qiagen, Paris, France), CD166 (Cat. No. Q100026824, Qiagen, Paris, France), Notch 1 (Cat. No. QT00001365, Qiagen, Paris, France), ACAN (Cat. No. QT00001365, Qiagen, Paris, France), Col II A1 (Cat. No. QT00049518, Qiagen, Paris, France).

Techniques: Isolation, Expressing, Control