Journal: Science Advances

Article Title: Tolerogenic nanoparticles induce type II collagen–specific regulatory T cells and ameliorate osteoarthritis

doi: 10.1126/sciadv.abo5284

Figure Lengend Snippet: ( A ) Proportion of OVA-specific T regs in iLN following intradermal injection of different types of nanoparticles to OT-II transgenic mice that have T cells specific for OVA only, as evaluated by flow cytometry ( n = 4). ( B ) Proportion of T regs generated in vitro from OT-II CD4 + T cells following coculture of the CD4 + T cells with DCs that had been treated with various types of nanoparticles in vitro ( n = 5). ( C ) Proportion of T regs in splenocytes. The splenocytes were isolated from wild-type OA mice that had been treated with intradermal injection of different types of nanoparticles and subsequently restimulated in vitro with Col II or OVA ( n = 5). ( D ) Relative mRNA expression of FOXP3 and TGF-β in the splenocytes restimulated in vitro with Col II or OVA ( n = 6). ( E ) Proportion of T regs in splenocytes analyzed by flow cytometry. The splenocytes were isolated from wild-type OA mice that had been treated with intradermal injection of different types of nanoparticles and subsequently restimulated in vitro with Col II 259–273 (epitope) or OVA ( n = 5). (A to E) * P < 0.05, ** P < 0.01, and *** P < 0.001. One-way ANOVA (multiple comparison) with Tukey’s post hoc test.

Article Snippet: LNP-Col II-R nanoparticles were labeled with DiR (AAT Bioquest, USA).

Techniques: Injection, Transgenic Assay, Flow Cytometry, Generated, In Vitro, Isolation, Expressing, Comparison